Cells, Free Full-Text

Long, noncoding RNAs (lncRNAs) are indispensable for normal cell physiology and, consequently, are tightly regulated in human cells. Yet, unlike mRNA, substantially less is known about the mechanisms for lncRNA degradation. It is important to delineate the regulatory control of lncRNA degradation, particularly for lncRNA telomeric repeat-containing RNA (TERRA), as the TERRA-telomere R-loops dictate cell cycle progression and genomic stability. We now report that the exosome complex component Exosc9 degrades lncRNA TERRA in human mammary epithelial cells. Heterochromatin protein 1 alpha (HP1α) recruits Exosc9 to the telomeres; specifically, the SUMO-modified form of HP1α supports interaction with Exosc9 and, as previously reported, lncRNA TERRA. The telomeric enrichment of Exosc9 is cell cycle-dependent and consistent with the loss of telomeric TERRA in the S/G2 phase. Elevated Exosc9 is frequently observed and drives the growth of endocrine therapy-resistant (ET-R) HR+ breast cancer (BCa) cells. Specifically, the knockdown of Exosc9 inversely impacts telomeric R-loops and the integrity of the chromosome ends of ET-R cells. Consistently, Exosc9 levels dictate DNA damage and the sensitivity of ET-R BCa cells to PARP inhibitors. In this regard, Exosc9 may serve as a promising biomarker for predicting the response to PARP inhibitors as a targeted monotherapy for ET-R HR+ BCa.

An integrated in vivo/in vitro framework to enhance cell-free

Postbiotics-parabiotics: the new horizons in microbial biotherapy

Remote immune processes revealed by immune-derived circulating

PDF) Protein structural biology using cell-free platform from

Cells, Free Full-Text

Anode‐Free Full Cells: A Pathway to High‐Energy Density Lithium

Improving cell-free glycoprotein synthesis by characterizing and

Scheme of cell free cloning using DMF. ( A ) The full length

Cell-free Macromolecular Synthesis

Mol Imaging Radionucl Ther on X: The Role of a Bone SPECT/CT Scan

Full-spectrum cell-free RAN for 6G systems: s

Textile-embedded cell-free biosensors

Cancer type classification using plasma cell-free RNAs derived

Cell Circuits and Complex Tissues